If AutoCal does not work for you then the RX offset between the transceiver and the openSPOT is greater than 500Hz. What you can do is to switch frequencies on the openSPOT, but keep the transceiver's frequency. For example, if the transceiver is on 433.900MHz, then test by setting the openSPOT to the following frequencies:
433900500 Hz
433900600 Hz
433900700 Hz
...
433899500 Hz
433899400 Hz
433899300 Hz
...
You can use the built-in local echo service to test your own voice on DMR ID 9999.